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DNA from cell lines

ECACC has developed a range of high quality, high molecular weight genomic DNA products derived from cell lines, which are available from stock. To ensure the highest quality and reproducibility, extracted DNA is subject to defined quality control procedures and all our DNA products are supplied at a standard concentration of 100ng/ul.

 
Cell line collection-derived DNA: DNA can be supplied in a range of standard quantities from catalogue listings - check individual product pages

Custom DNA Extraction: DNA can be prepared from your own cell lines and blood samples as part of our Human Genetic Services

DNA Panels: A range of off the shelf DNA products in a convenient 8 x 12 format are available. These include:

High quality, high molecular weight genomic DNA is extracted from cell lines using an automated extraction unit employing silica coated magnetic bead technology. The DNA is quantified using the PicoGreen® fluorescence method for accurate reproducible results.


ECACC is accredited to International Quality Standard ISO 9001:2008, and all procedures are managed in accordance with the requirements of the standard. To ensure the highest quality, all ECACC DNA products are subjected to defined quality control procedures which include:


DNA size and integrity
Agarose gel electrophoresis is used to verify the high molecular weight and integrity of the DNA. Our DNA extraction procedure consistently produces genomic DNA between 20-30Kb in size.

Purity
UV spectrophotometry at 260/280nm and 230/260nm wavelength ratios is used to determine purity of the extracted DNA.

Authenticity
Short tandem repeat (STR) PCR, using a commercially available forensic kit consisting of 10 markers plus amelogenin, is used to verify the identity of each batch of DNA against source material.

Performance trials
The performance of ECACC DNA products has been independently assessed using a selection of assays. These include the 5' nuclease assay (also known as the Taqman assay), HLA typing, and STR PCR.

Long term stability and performance trials
ECACC has performed stability and performance trials on samples of DNA from each of the Human Random Control DNA panels over several years. This involves repeated agarose gel electrophoresis and repeated use as a template for PCR analysis. No reduction of PCR product has been observed in tests on samples stored at 4°C for up to 28 months.

DNA products are supplied at either +4°C or frozen. Please consult the data sheet which accompanies the delivery; these sheets contain valuable information about how the DNA should be handled and stored. We recommend that frozen DNA products be stored at -20°C upon receipt if not to be used immediately. Repeated freeze thawing should be avoided.

On receipt of your DNA order ECACC recommends the following:

DNA received in tubes

  1. Once thawed the DNA can be stored at 4°C to 8°C for at least six months

  2. Before unscrewing the cap of the tube, place in a mini centrifuge for a short spin to bring all DNA to the bottom of the tube

  3. Take care not to mix caps between samples. If in doubt use a fresh cap.

DNA received in 96 well plates

  1. Once the plates have been thawed, prior to piercing, they should be centrifuged briefly to bring all DNA to the bottom of the well

  2. DNA supplied in 96 well plates is designed for single use. However, should it be necessary to store the plates then the plastic lid should be placed over the pierced wells. The plate should be stored upright at 4°C to 8°C. The DNA will evaporate very quickly (within days) if the plate is not re-sealed using the replacement seal supplied. When re-sealed, the DNA can be stored for a minimum of three months at 4°C to 8°C

  3. ECACC does not recommend that you re-freeze the plates but, if you do, the plates should be re-sealed and stored upright.

 

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